四、結論

油和/或 Corexit 的存在會導致 EPS 的蛋白質：多糖比率更高，并在中胚層實驗中降低 SFT。 在這些實驗中，SFT 與 蛋白質：具有負斜率的 EPS 多糖。 當開闊的海洋 水域和兩種不同的沿海水處理進行了比較， 蛋白質趨勢：多糖為 CEWAF > DCEWAF > WAF ≥ Control 并且對于 SFT，它是相反的， CEWAF < DCEWAF < WAF ≤ 對照。 因此，SFT 與膠體 EPS 中的蛋白質：多糖比率成反比。

當中宇宙水柱的不同尺寸分數為 相比之下，我們發現 EPS 膠體可以降低 SFT 蛋白質：多糖比例，表明有效的生物乳化 蛋白質的容量。 粒子濾波中 SFT 的比較 分數 (< 0.45 μm) 和 EPS 膠體分數 (< 0.45 μm 和 > 3 kDa)，對于真正溶解的部分 (< 3 kDa)，它是 表明只有前兩個包含 EPS 的部分具有容量 以降低 SFT，而 < 3 kDa 級分顯示與以下相同的 SFT 純海水或只有真正溶解有機碳的海水。

顯微鏡技術（即 CLSM 和 SEM）證實，正如預測的那樣，蛋白質主要在空氣 - 水界面富集， 強烈影響空氣/水界面處的 SFT 治療。 這些技術還可視化了不同的聚集體尺寸 和它們的分散，以及聚集體形成的重要性 通過陰離子EPS組分部分之間的Ca2+"橋接"。 SFT 可能會發生微小的變化，與蛋白質：多糖比率的變化相吻合，這可能是 pH 值變化的原因（十分之一） 單位），如 EPS 模型化合物所示，這可能在 CMC 周圍最為突出。 此外，我們表明蛋白質和酸性多糖的 EPS 模型成分比 Corexit 導致海水中膠束的自組裝甚至 當這些成分的濃度很低時。 這個 表明 EPS 在形成方面與 Corexit 相同或更有效 乳液。 然而，關于相互作用的更系統的研究 不同組件的不同組合，以及更多型號 單獨的化合物，可能需要更多地闡明在我們的中宇宙實驗中觀察到的復雜性。

致謝

這項研究得到了墨西哥灣的資助 支持名為 ADDOMEx 的聯盟研究的研究計劃 (微生物對分散劑和油的聚集和降解 Exopolymers) 聯盟。 原始數據可以在海灣找到 墨西哥研究倡議信息和數據合作組織 (GRIIDC) 在網址 https://doi.org/10.7266/N7PK0D64； https://doi.org/10。 7266/N78P5XZD； https://doi.org/10.7266/N74X568X； https://doi. org/10.7266/N79W0D1K。

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蛋白質外聚物中多糖的比例——摘要、簡介

蛋白質外聚物中多糖的比例——方法

蛋白質外聚物中多糖的比例——結果與討論

蛋白質外聚物中多糖的比例——結論、致謝！